Darkfield microscope

From Freepedia

A Darkfield microscope uses a method whereby the sample being viewed is actually infront of a dark background and light is being angled onto the specimen from the sides.

stains cannot be used in this method as the specimen-cell becomes dead during the staining.in dark field microscopy light is prevented from entering the central region by placing a disc in the condensor.thus the main field of obsevation becomes dark.

the light entering from the peripheral region of the condenser becomes refracted and reflected.

the technique is specifically useful in the study of mitochondria,plastids,nucleus,etc.