Primer walking

From Freepedia

Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. The DNA of interest may be either a plasmid insert or a PCR product. The initial sequencing will be performed from each end using either universal primers or primers designated by the customer. In order to completely sequence the region of interest design and synthesis of new primers is necessary to obtain contiguous sequence information.