Topoisomerase
From Freepedia
Topoisomerases (type I: EC 5.99.1.2, type II: EC 5.99.1.3) are enzymes that act on the topology of DNA. The double-helical configuration that DNA strands naturally reside in makes them difficult to separate, and yet they must be separated by helicase proteins if other enzymes are to transcribe the sequences that encode proteins, or if chromosomes are to be replicated. In so-called circular DNA, in which double helical DNA is bent around and joined in a circle, the two strands are topologically linked, or knotted. Otherwise identical loops of DNA having different numbers of twists are topoisomers, and cannot be interconverted by any process that does not involve the breaking of DNA strands. Topoisomerases catalyze and guide the unknotting of DNA.
The insertion of viral DNA into chromosomes and other forms of recombination can also require the action of topoisomerases.
Many drugs operate through interference with the topoisomerases. The broad-spectrum fluoroquinolone antibiotics act by disrupting the function of bacterial type II topoisomerases. Some chemotherapy drugs work by interfering with topoisomerases in cancer cells: type 1 is inhibited by irinotecan and topotecan, while type 2 is inhibited by etoposide and teniposide.
Type I topoisomerases
Both type I and type II topoisomerases change the supercoiling of DNA. Type I topoisomerases function by nicking one of the strands of the DNA double helix, twisting it around the other strand, and religating the nicked strand. Type I topoisomerases change the linking number of a circular DNA strand by 1. Type I enzymes can be further subdivided into type IA and type IB, based on their chemistry of action. All topoisomerases form a phosphotyrosine intermediate between the catalytic tyrosine of the enzyme and the scissile phosphoryl of the DNA backbone. Type IA topoisomerases form a covalent linkage between the catalytic tyrosine and the 5'-phosphoryl while type IB enzymes form a covalent 3'-phosphotyrosine intermediate.
Type II topoisomerases
Type II topoisomerases cut both strands of the DNA helix simultaneously. Once cut, the ends of the DNA are separated, and a second DNA duplex is passed through the break. Following passage, the cut DNA is resealed. This reaction allows type II topoisomerases to change the linking number of a DNA loop by +/-2, and promotes chromosome disentanglement. For example, DNA gyrase, a type II isomerase observed in E. coli and most other prokaryotes, introduces negative supercoils and decreases the linking number by 2. Gyrase also is able to remove knots from the bacterial chromosome. There are two subclasses of type II topoisomerases, type IIA and IIB. Type IIA topoisomerases include the enzymes DNA gyrase, eukaryotic topoisomerase II, and bacterial topoisomerase IV. Type IIB topoisomerases are structurally and biochemically distinct, and comprise a single family member, topoisomerase VI. Type IIB topos are found in archaea and some higher plants.
